Since 2016, the Electron Microscopy (EM) facility provides advanced expertise in electron cryomicroscopy, from sample preparation to image analysis, with a focus on single particle analysis.

The equipment is maintained and managed by the facility staff, who also provide training and assistance for anyone wishing to use any of the equipment listed below. Cryo-EM facilities are available for internal users and on a collaborative basis. Please get in touch with Sarah Schneider to discuss possible access.

Equipment

Vitrobot Mark IV and PELCO easiGlow glow discharger

Edwards carbon evaporator

The T12 microscope is used for sample screening before data collection on a high-end microscope such as the Glacios or Titan Krios.

Tecnai G2 Spirit BT (T12):

120 kV accelerating voltage, LaB6 source. 16M CETA camera, used mainly for negative staining samples, but also capable of cryo-EM using side entry cryo-holders.

200kV Glacios microscope equipped with Field Emission Gun and Falcon 3EC direct electron detector.

Glacios:

200 kV accelerating voltage, field emission gun (X-FEG) source. This microscope is equipped with an autoloader, Falcon 3EC direct detector and is connected to WARP on-the-fly data processing. This microscope is used for cryo-EM sample optimisation and data collection.

Data storage:

Recently, EMBL Grenoble made a specia storage system investment to accommodate the ever expanding EM data for the 5 next years. The new system will be run using NetApp technology. This investment will be complemented in 2021 with a high performance storage system that will reduce EM calculation processing time.

High-performance computing cluster:

The computing cluster shared with the Institut de Biologie Structurale (IBS) allows efficient data processing using state-of-the-art data processing software such as Relion or CryoSPARC. Eight cluster nodes comprising a total of 40 GPUs allow efficient data processing by multiple users. CPU cluster with 600 CPU provides additional resources for other software packages. High-performance computing at EMBL is supported by SBGrid.

Recent achievements

  1. Bhogaraju et al., Nature 2019.Structure of SidJ/Cam complex determined to 4.1A from the Glacios microscope.
  2. Wandzik et al., A Structure-Based Model for the Complete Transcription Cycle of Influenza Polymerase. Cell. 2020 181(4):877-893.e21. doi: 10.1016/j.cell.2020.03.061

EM Team

Sarah Schneider
Facility manager responsible for all operations

Michael Hons
Cryo-EM staff scientist working at ESRF CM01 beamline

Wojtek Galej
Group leader in charge

Collaboration

EMBL Grenoble also participates, together with the other Partnership for Structural Biology (PSB) institutes, in running the CM01 Titan Krios cryo-electron microscope at the ESRF for external users.

For more information regarding access, you can visit the ESRF page or contact EMBL staff scientist Michael Hons.